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C-CBL-Driven SRC Ubiquitination Intrachain TR-FRET Assay Kit

  • 產(chǎn)品型號(hào)：78822
  • 廠商性質(zhì)：生產(chǎn)廠家
  • 更新時(shí)間：2023-11-12
  • 訪  問(wèn)  量：204
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Description

The C-CBL-driven SRC Ubiquitination Intrachain TR-FRET Assay Kit utilizes a Europium cryptate-labeled Ubiquitin Donor and a Cy5-labeled Ubiquitin Acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, this assay does not detect mono-ubiquitination. The FRET-based format requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses of polyubiquitination.

Figure 1: C-CBL-driven SRC ubiquitination intrachain TR-FRET Assay Kit schematic.

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Synonyms
E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, RING-type E3 ubiquitin transferase CBL, Signal transduction protein CBL, CBL, CBL2, RNF55, Proto-oncogene c-Src, pp60c-src (p60-Src)
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