The DOT1L Chemiluminescent Assay Kit is designed to measure DOT1L activity for screening and profiling applications. The DOT1L Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate, DOT1L substrate, primary antibody against methylated lysine residue of Histone H3, secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer, and purified DOT1L for 100 enzyme reactions. The key to the DOT1L Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K79 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for two hours. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

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