The Mouse PDE1C Assay Kit is designed for identification of inhibitors of Mouse PDE1C using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE1C to the binding agent.

The Mouse PDE1C Assay Kit comes in a convenient 96-well format, with purified Mouse PDE1C enzyme, fluorescently labeled substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the Mouse PDE1C Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE1C reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE1C for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.