The STAT5 (Signal transducer and activator of transcription 5) Luciferase Reporter U937 cell line monitors STAT5-mediated signal transduction pathways in the human monocyte cell line U937. It contains a firefly luciferase gene driven by STAT5 response elements located upstream of the minimal TATA promoter. After activation by GM-CSF (Granulocyte-macrophage colony-stimulating factor), the endogenous transcription factor STAT5 binds to the response elements, inducing transcription of the luciferase reporter gene.

These cells respond to GM-CSF. Functional validation experiments showed that GM-CSF-induced luciferase activity was inhibited by anti-GM-CSF neutralizing antibody (Clone #BVD2-21C11).

Figure 1: Illustration of STAT5 Luciferase Reporter U937 Cell Line mechanism of action.
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